TY - JOUR AU - Perdomo-Celis, Federico AU - Salgado, Doris Marta AU - Narváez, Carlos Fernando PY - 2016/01/02 Y2 - 2024/03/29 TI - Development of a capture Elisa for the detection of dengue-specific immunoglobulin M JF - RFS Revista Facultad de Salud JA - RFS VL - 8 IS - 1 SE - DO - 10.25054/rfs.v8i1.1317 UR - https://journalusco.edu.co/index.php/rfs/article/view/1317 SP - 9 - 15 AB - <span>Dengue is an important viral vector-borne disease worldwide. Its diagnosis requires laboratory confirmation and the detection of dengue virus (DENV)-specific immunoglobulin (lg) M by capture enzyme-linked immunosorbent assay (ELISA) is a method frequently used. However, commercial kits of IgM-capture ELISA for dengue diagnosis are expensive not easily available and obtain the results take long time in endemic areas. Here, we aimed to standardize an in-house capture ELISA for the detection of DENV-specific IgM in plasma for diagnosis of DENV infection. Plasma from two children with confirmed acute DENV-2 infection was evaluated and two mouse monoclonal anti-DENV antibodies (one type-specific and the other one serotype-cross-reactive) were tested. The assay was effective in the detection of DENV-specific IgM in plasma and was comparable with a commercially available kit, even at the highest dilutions of the sample and the mouse anti-DENV antibodies. Furthermore, in a child with primary DENV-2 infection, plasma anti-DENV IgM was found to be serotype-specific. This work strengthens the technical capacity for the study and diagnosis of DENV infection in an endemic area.</span> ER -