Desarrollo de una captura de Elisa para la detección de dengue-inmunoglobulina específica M

Federico Perdomo-Celis; Doris Marta Salgado; Carlos Fernando Narváez

doi:10.25054/rfs.v8i1.1317

Development of a capture Elisa for the detection of dengue-specific immunoglobulin M

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Perdomo-Celis, F., Salgado, D. M., & Narváez, C. F. (2016). Development of a capture Elisa for the detection of dengue-specific immunoglobulin M. RFS Revista Facultad De Salud, 8(1), 9–15. https://doi.org/10.25054/rfs.v8i1.1317

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Published: Jan 2, 2016

Doi

https://doi.org/10.25054/rfs.v8i1.1317

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PlumX

Issue

Vol. 8 No. 1 (2016)

Section

Artículos de investigación

Federico Perdomo-Celis Universidad Surcolombiana

Doris Marta Salgado Universidad Surcolombiana

Carlos Fernando Narváez Universidad Surcolombiana

Abstract

Dengue is an important viral vector-borne disease worldwide. Its diagnosis requires laboratory confirmation and the detection of dengue virus (DENV)-specific immunoglobulin (lg) M by capture enzyme-linked immunosorbent assay (ELISA) is a method frequently used. However, commercial kits of IgM-capture ELISA for dengue diagnosis are expensive not easily available and obtain the results take long time in endemic areas. Here, we aimed to standardize an in-house capture ELISA for the detection of DENV-specific IgM in plasma for diagnosis of DENV infection. Plasma from two children with confirmed acute DENV-2 infection was evaluated and two mouse monoclonal anti-DENV antibodies (one type-specific and the other one serotype-cross-reactive) were tested. The assay was effective in the detection of DENV-specific IgM in plasma and was comparable with a commercially available kit, even at the highest dilutions of the sample and the mouse anti-DENV antibodies. Furthermore, in a child with primary DENV-2 infection, plasma anti-DENV IgM was found to be serotype-specific. This work strengthens the technical capacity for the study and diagnosis of DENV infection in an endemic area.

Keywords

Dengue

ELISA

plasma

immunoglobulin M

cross-reactive

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Author Biographies / See

Federico Perdomo-Celis, Universidad Surcolombiana

Semillero de formación SINEDIR, Grupo de Parasitología y Medicina Tropical, Programa de Medicina, Universidad Surcolombiana, Neiva, Colombia.

Doris Marta Salgado, Universidad Surcolombiana

Semillero de formación SINEDIR, Grupo de Parasitología y Medicina Tropical, Programa de Medicina, Universidad Surcolombiana, Neiva, Colombia. Departamento de Pediatría, Hospital Universitario de Neiva, Colombia

Carlos Fernando Narváez, Universidad Surcolombiana

Semillero de formación SINEDIR, Grupo de Parasitología y Medicina Tropical, Programa de Medicina, Universidad Surcolombiana, Neiva, Colombia.

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