Evaluation of the antimicrobial activity of lactic acid bacteria isolated from artisanal and commercial dairy products against Escherichia coli
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Lactic acid bacteria (LAB) stand out for their ability to produce substances with antimicrobial activity such as organic acids, hydrogen peroxide and bacteriocins, therefore, the isolation and identification of LAB strains was conducted from yoghurt and cheese of artisanal or commercial elaboration from the Huila Department of Colombia.
Once isolated antimicrobial activity was evaluated against strains of pathogenic Escherichia coli (isolated from restaurant Surcolombiana University and a peddling aji. using different methods: presence of cells (disk method), absence of cells (well method) with neutral and acid pH. The results obtained in the antimicrobial activity by the BAL, opens the possibility of offering to the local semi-industrial, artisanal dairy industry and to consumers, information that will help them to produce food (Yogurt and Quesillo) with attributes of quality, and probiotic potential with antimicrobial activity, positioning them as a functional food. The results of the physical and chemical characterization of dairy products (Yogurt and Quesillo), elaborated and distributed in an artisanal and semi-industrial way in the department of Huila, are of great relevance not only to guarantee their quality and acceptability in the market for the consumers; but also as a useful identity tool to classify the different types of quesillos and yogurts.
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Aiba, Y.; Nobuyuki, S.; Kabir, A.M.; TakagI, A.; Koga, Y. 1998. Lactic acid-mediated suppression of helicobacter pylori by the oral administration of lactobacillus salivarus as a probiotic in a gnotobiotic murine model. The American Journal of gastroenterology, págs.2097-2101.
Amorocho, C. M. 2011. Caracterización y potencial probiótico de bacterias lácticas aisladas de leche de oveja Guirra. Valencia: Universidad Politecnica de Valencia.
AOAC (Association of Official Analytical Chemists), 1997. Methods of Analysis of the AOAC International. 3rd ed. Volumen II. Maryland USA.
Castillo, J. 2001. Elaboración de queso mozzarella con diferentes porcentajes de grasa en la leche de vaca. Trabajo de grado de Ingeniería 75 agrónoma. Guácimo. Costa Rica.: Universidad EARTH. Facultad de Ingeniería agronómica. Pag. 36.
Cortes, E.T.;Peña, N.; Amorocho, C.M. y Gutierrez, N. 2016. Evolucion de parámetros fisicoquímicos de quesillo Huilense, en almacenamiento refrigerado, Biotecnologia en el sector agropecuario y agroindustrial, Vol 14 N°2, pág 110-118.
FAO (Food and Agriculture Organization). 2003. Norma del Codex para Leches Fermentadas: CODEX STAN 243-2003. Leche y Productos Lácteos (2da edición). GARCÍA M. E M. & Fernandez S. I. 2012. Determinación de la humedad de un alimento por un método gravimétrico indirecto por desecación.
http://riunet.upv.es/bitstream/handle/10251/16339/Determinaci%C3%B3n20de%20humedad. df?sequence=1&isAllowed=y. (Consultado: Junio de 2016).
Gonzales, E.P. 2010. Caracterización de la composición fisicoquímica del queso fresco elaborado artesanalmente en Sehualaca municipio de Minatitlan, Veracruz. Tesis Médico veterinario Zootecnista, Universidad Veracruzana,52 p.
Hudault, S., Liévin, V., Oise Bernet-Camard, M.F., Servin, A.L. 1997. Antagonistic Activity Exerted in vitro and in vivo by Lactobacillus casei (Strain GG) against Salmonella typhimurium C5 Infection. Applied and Environmental Microbiology, págs.513-518.
Jacobsen, C.N., Nielsen, V.R., Hayford, A.E., Moller, P.L., Michaelsen, K.F., Paerregaard, A., Sandström, B., Tvede, M., Jakobsen, M. 1999. Screening of Probiotic Activities of forty seven strains of Lactobacillus spp. By In vitro techniques and evaluation of the colonization ability of five selected strain in humans. Applied and Environmental Microbiology, 65(11), págs.4949-4956.
Jamuna M.; Jeevaratnam, K. 2004. “Isolation and characterization of lactobacilli from some traditional fermented foods and evaluation of the bacteriocins” J Gen Appl Microbiol 50: 79–90.
Maldonado, R. y Llanca L. 2008. Estudio de la calidad del queso de mano comercializado en el municipio de Girardot, estado Aragua. Venezuela. Revista Científica v.18 N.4. Maracaibo.
Midolo, P.D.; Lambed, J.R.; Hull, R.; Luo, F. y Grayson, M.L. 1995. In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria. Journal of Applied Bacteriology, 79, págs.475-479.
Oliszewski, R.; Cisint, J. C. y Kairúz, M. 2007. Manufacturing characteristics and shelf life of Quesillo, an Argentinean traditional cheese. Food control, 18(6), 736-741.
Olivares, M.; Díaz-Ropero, M.P.; Martín, R.; Rodríguez, J.M. y Xaus, J. 2006. Antimicrobial potential of four Lactobacillus strains isolated from breast milk. Journal of Applied Microbiology, 101(1), págs.72-79.
Pérez, M.A. y Echeverry, S. 2015. Análisis microbiológico de alimentos preparados en la vía pública en los alrededores de la universidad surcolombiana mediante el estudio de coliformes. Tesis de pregrado en Licenciatura de Ciencias Biológicas, Universidad Surcolombiana, Neiva, Colombia. 190 p.
Ramírez, J. y Rodríguez, A. 2012. Caracterización del quesillo colombiano por espectrocolorimetría. Vitae, vol. 19, No. 2, pp.178-185.
Ramírez, C. y Vélez, J.F. 2012. Quesos frescos: propiedades, métodos de determinación y factores que afectan su calidad.
Schimidt, C.A.P.; Pereira, C.; Dos Anjos, G. y Lucas, S.D.M. 2012. Formulação e avaliação sensorial hedônica de iogurte com polpa de Acerola. Revista Eletrônica Científica Inovação e Tecnologia Vol.1 (5):pag 10-14.
Yuan, K.L. 2006. Microbial biotechnology. Principles and applications Second., National University of Singapore.